BS ISO BRITISH STANDARD 23065:2009 Milk fat from enriched dairy products - Determination of omega-3 and omega-6 fatty acid content by gas-liquid chromatography ICS 67.100.10 BSi NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW British Standards BSISO23065:2009 National foreword This British Standard is theUK implementation of ISO23065:2009. The UK participation in its preparation was entrusted to Technical Committee AW/5, Chemical analysis of milk and milk products. Alist of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard Amendments/corrigendaissuedsincepublication was published under the authority ofthe Standards Policy and Strategy Date Comments Committee on 31 October 2009 BSI 2009 ISBN 978 0 580 55197 0 BS ISO 23065:2009 ISO INTERNATIONAL STANDARD 23065 IDF 211 First edition 2009-03-01 Milk fat from enriched dairy products - Determination of omega-3 and omega-6 fatty acid content by gas-liquid chromatography Matiere grasse laitiere deproduits laitiers enrichis-Determination de gaz-liquide Reference numbers ISO 23065:2009(E) IDF 211:2009(E) @ISOandIDF2009 BSISO23065:2009 ISO23065:2009(E) IDF 211:2009(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. Neither the ISO Central Secretariat nor the IDF accepts any liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by Iso member bodies and IDF national committees. In the unlikely event that a problem relating to it is found, please inform the ISO Central Secretariat at the address given below. COPYRIGHTPROTECTEDDOCUMENT ISO and IDF 2009 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO or IDF at the respective address below. ISO copyright office Intemational Dairy Federation Case postale 56.CH-1211Geneva 20 Diamant Building : Boulevard Auguste Reyers 80·B-1030 Brussels Tel. + 4122 749 0111 Tel. + 32 2 733 98 88 Fax + 41 22 749 09 47 Fax + 32 2 733 04 13 E-mail [email protected] E-mail [email protected] Web www.iso.org Web www.fil-idf.org Published in Switzerland ii ISO and IDF 2009 - All rights reserved BSISO23065:2009 ISO23065:2009(E) IDF211:2009(E) Contents Page Foreword.. iv Foreword 1 Scope 2 Normative references.. 3 Termsand definitions. 4 Principle. 5 Reagents 6 Apparatus 7 Sampling.. 8 Preparation of test sample.. 5 9 Procedure... 9.1 Calibrationsolution forthedeterminationof LA, EPAandDHAresponsefactor 9.2 Test portion.......... 9.3 Qualitative determination... 9.4 Quantitativedetermination 9.5 Calculationandexpressionofresults 9.6 Expressionofresults 10 Precision 10.1 Repeatability.. 10.2 Reproducibility. 11 Test report.. Annex A (informative)Examples ofgas-liquid chromatographic analysis AnnexB (informative)Interlaboratorytrial.... Bibliography.... @ ISO and IDF 2009 -All rights reserved ili BSISO 23065:2009 ISO23065:2009(E) IDF 211:2009(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the ru